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7aad  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec 7aad
    7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aad/7-AAD+Staining+Solution/us12698315-681-21-22
    Average 97 stars, based on 81 article reviews
    7aad - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Enhanced bacterial cancer therapy delivering therapeutic RNA interference of c-Myc
    Article Snippet: .. Isolated PBMCs were stained with 7-AAD and APC-conjugated anti-CCL2 antibody (Miltenyi Biotec, UK; #130-107-483, clone REA485). .. Samples were analysed using a BD LSRFortessa™ Cell Analyzer.

    Staining:

    Article Title: Enhanced bacterial cancer therapy delivering therapeutic RNA interference of c-Myc
    Article Snippet: .. Isolated PBMCs were stained with 7-AAD and APC-conjugated anti-CCL2 antibody (Miltenyi Biotec, UK; #130-107-483, clone REA485). .. Samples were analysed using a BD LSRFortessa™ Cell Analyzer.

    Article Title: Identification and Characterization of Fully Human FOLR1-Targeting CAR T Cells for the Treatment of Ovarian Cancer.
    Article Snippet: .. On the day of assay use, the number of viable CAR T cells was determined by staining them with 7-AAD, biotinylated human FOLR1 Protein (Miltenyi Biotec, Bergisch Gladbach, Germany), and an anti-biotin antibody conjugated to PE. .. Human embryonic kidney 293T cells (HEK293T, ACC 635) were obtained from the DSMZ—German Collection of Microorganisms and Cell Cultures.

    Article Title: HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia
    Article Snippet: In total, 5 μL 7-AAD (BD Biosciences) was added to each sample, and samples were incubated for 15 minutes. .. Staining for 7-AAD was quantified using flow cytometry (MACSQuant, Miltenyi Biotec), using unstained cells to set the positive gate. ..

    Article Title: Feeder-cell-free system for ex vivo production of natural killer cells from cord blood hematopoietic stem and progenitor cells.
    Article Snippet: .. For surface staining, cells were incubated with 7- AAD (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 15 to 45 min at 4°C, washed and resuspended in FACS buffer (0.5% BSA Fraction V (EuroBio Scientific, Les Ulis, France) and 2mM EDTA (ThermoFisher Scientific, Dardilly, France) in 1X PBS (Eurobio Scientific, Les Ulis, France). .. For intracellular stainings, cells were first surface stained with ViobilityTM Fixable Dye (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 30min in ice and washed with FACS buffer.

    Article Title: Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD.
    Article Snippet: .. Cytotoxicity of various forms of anti-CD3 Abs against expanded and Unact T cell. (A) Illustration of the different forms of Abs used in this study. (B) Seven-day expanded human T cells were incubated with UCHT1-IgG or UCHT1-scFv (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and shown as a flow cytometry plot. (C) Human T cells purified from PBMCs by MACS using mixed CD4 and CD8 microbeads (Unact T cell, Miltenyi Biotec) were incubated with different forms of OKT3 (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and presented as a flow cytometry plot. ..

    Article Title: HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia.
    Article Snippet: In total, 5 μL 7-AAD (BD Biosciences) was added to each sample, and samples were incubated for 15 minutes. .. Staining for 7-AAD was quantified using flow cytometry (MACSQuant, Miltenyi Biotec), using unstained cells to set the positive gate. ..

    Article Title: Identification and Characterization of Fully Human FOLR1-Targeting CAR T Cells for the Treatment of Ovarian Cancer
    Article Snippet: .. On the day of assay use, the number of viable CAR T cells was determined by staining them with 7-AAD, biotinylated human FOLR1 Protein (Miltenyi Biotec, Bergisch Gladbach, Germany), and an anti-biotin antibody conjugated to PE. .. Human embryonic kidney 293T cells (HEK293T, ACC 635) were obtained from the DSMZ—German Collection of Microorganisms and Cell Cultures.

    Flow Cytometry:

    Article Title: Somatic Mutations of Thymic Epithelial Tumors Identified in the Prospective THYMOGENE Trial
    Article Snippet: The samples were then incubated on an Eppendorf ThermoMixer C thermoblock for 45 min at 37°C at 350 RPM and then filtered using a MACS SmartStrainer 100 μm (Miltenyi Biotec). .. Flow cytometry was performed using the MACSQUANT Analyzer 10 Flow Cytometer (Miltenyi Biotec), employing 7‐AAD, CD3‐VioBlue, CD45‐APC, and CD326‐PE (Miltenyi Biotec). .. The data were analyzed using MACSQuantify Software (Miltenyi Biotec).

    Article Title: HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia
    Article Snippet: In total, 5 μL 7-AAD (BD Biosciences) was added to each sample, and samples were incubated for 15 minutes. .. Staining for 7-AAD was quantified using flow cytometry (MACSQuant, Miltenyi Biotec), using unstained cells to set the positive gate. ..

    Article Title: Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD.
    Article Snippet: .. Cytotoxicity of various forms of anti-CD3 Abs against expanded and Unact T cell. (A) Illustration of the different forms of Abs used in this study. (B) Seven-day expanded human T cells were incubated with UCHT1-IgG or UCHT1-scFv (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and shown as a flow cytometry plot. (C) Human T cells purified from PBMCs by MACS using mixed CD4 and CD8 microbeads (Unact T cell, Miltenyi Biotec) were incubated with different forms of OKT3 (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and presented as a flow cytometry plot. ..

    Article Title: HTATIP2 regulates arteriogenic activity in monocytes from patients with limb ischemia.
    Article Snippet: In total, 5 μL 7-AAD (BD Biosciences) was added to each sample, and samples were incubated for 15 minutes. .. Staining for 7-AAD was quantified using flow cytometry (MACSQuant, Miltenyi Biotec), using unstained cells to set the positive gate. ..

    Incubation:

    Article Title: Feeder-cell-free system for ex vivo production of natural killer cells from cord blood hematopoietic stem and progenitor cells.
    Article Snippet: .. For surface staining, cells were incubated with 7- AAD (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 15 to 45 min at 4°C, washed and resuspended in FACS buffer (0.5% BSA Fraction V (EuroBio Scientific, Les Ulis, France) and 2mM EDTA (ThermoFisher Scientific, Dardilly, France) in 1X PBS (Eurobio Scientific, Les Ulis, France). .. For intracellular stainings, cells were first surface stained with ViobilityTM Fixable Dye (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 30min in ice and washed with FACS buffer.

    Article Title: Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD.
    Article Snippet: .. Cytotoxicity of various forms of anti-CD3 Abs against expanded and Unact T cell. (A) Illustration of the different forms of Abs used in this study. (B) Seven-day expanded human T cells were incubated with UCHT1-IgG or UCHT1-scFv (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and shown as a flow cytometry plot. (C) Human T cells purified from PBMCs by MACS using mixed CD4 and CD8 microbeads (Unact T cell, Miltenyi Biotec) were incubated with different forms of OKT3 (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and presented as a flow cytometry plot. ..

    FACS:

    Article Title: Feeder-cell-free system for ex vivo production of natural killer cells from cord blood hematopoietic stem and progenitor cells.
    Article Snippet: .. For surface staining, cells were incubated with 7- AAD (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 15 to 45 min at 4°C, washed and resuspended in FACS buffer (0.5% BSA Fraction V (EuroBio Scientific, Les Ulis, France) and 2mM EDTA (ThermoFisher Scientific, Dardilly, France) in 1X PBS (Eurobio Scientific, Les Ulis, France). .. For intracellular stainings, cells were first surface stained with ViobilityTM Fixable Dye (Miltenyi Biotech, Bergisch Gladbach, Germany) and the appropriate antibodies for 30min in ice and washed with FACS buffer.

    Purification:

    Article Title: Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD.
    Article Snippet: .. Cytotoxicity of various forms of anti-CD3 Abs against expanded and Unact T cell. (A) Illustration of the different forms of Abs used in this study. (B) Seven-day expanded human T cells were incubated with UCHT1-IgG or UCHT1-scFv (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and shown as a flow cytometry plot. (C) Human T cells purified from PBMCs by MACS using mixed CD4 and CD8 microbeads (Unact T cell, Miltenyi Biotec) were incubated with different forms of OKT3 (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and presented as a flow cytometry plot. ..

    Magnetic Cell Separation:

    Article Title: Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD.
    Article Snippet: .. Cytotoxicity of various forms of anti-CD3 Abs against expanded and Unact T cell. (A) Illustration of the different forms of Abs used in this study. (B) Seven-day expanded human T cells were incubated with UCHT1-IgG or UCHT1-scFv (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and shown as a flow cytometry plot. (C) Human T cells purified from PBMCs by MACS using mixed CD4 and CD8 microbeads (Unact T cell, Miltenyi Biotec) were incubated with different forms of OKT3 (12.5 pmol/1×105 cells) for 24 h. Apoptotic cells were analyzed by staining with 7-AAD and Annexin V and presented as a flow cytometry plot. ..



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